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Felipe F.B. Tuon Khaiany Lino Florencio Clóvis Arns da Cunha Jaime Luis Lopes Rocha 《Revista iberoamericana de micología》2018,35(2):63-67
Background
Posaconazole is used for the prophylaxis of invasive fungal disease (IFD). Previous studies have shown it to be cost-effective compared to fluconazole/itraconazole. However, posaconazole has never been economically evaluated in developing countries.Aims
The aim of the present study was to perform a cost-effectiveness analysis of posaconazole compared to fluconazole in public (SUS) and private hospitals (PHS) in Brazil.Methods
A cost-effectiveness simulation was conducted on the basis of a pivotal study on the use of posaconazole in acute myeloid leukemia (AML) patients, adjusting the costs to Brazilian data.Results
A pharmacoeconomic analysis was performed on a hypothetical sample of 100 patients in each drug group. The total cost of posaconazole use alone was USD$ 220,656.31, whereas that for fluconazole was USD$ 83,875.00. Our results showed that patients with IFD remain hospitalized for an additional 12 days, at an average cost of USD$ 850.85 per patient per day. The total money spent by PHS for 100 patients for 100 days was USD$ 342,318.00 for the posaconazole group and USD$ 302,039.00 for the fluconazole group. An analysis of sensitivity (10%) revealed no intergroup difference.Conclusions
In Brazil posaconazole is cost-effective, and should be considered for the prophylaxis of patients with AMD/myelodysplasia (AML/MDS) undergoing chemotherapy. 相似文献13.
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Martínez-Sernández V Muiño L Perteguer MJ Gárate T Mezo M González-Warleta M Muro A Correia da Costa JM Romarís F Ubeira FM 《PLoS neglected tropical diseases》2011,5(11):e1376
Background
Human fasciolosis is a re-emerging disease worldwide and is caused by species of the genus Fasciola (F. hepatica and F. gigantica). Human fasciolosis can be diagnosed by classical coprological techniques, such as the Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection of acute infections, ectopic infections, or infections with low number of parasites. In such cases immunological methods may be a good alternative and are recommended for use in major hospitals where trained personnel are available, although they are not usually implemented for individual testing.Methodology/Principal Findings
We have developed a new lateral flow test (SeroFluke) for the serodiagnosis of human fasciolosis. The new test was constructed with a recombinant cathepsin L1 from F. hepatica, and uses protein A and mAb MM3 as detector reagents in the test and control lines, respectively. In comparison with an ELISA test (MM3-SERO) the SeroFluke test showed maximal specificity and sensitivity and can be used with serum or whole blood samples.Conclusions/Significance
The new test can be used in major hospitals in hypoendemic countries as well as in endemic/hyperendemic regions where point-of-care testing is required. 相似文献15.
Nestedness has been widely used to measure the structure of biological communities and occurs when species-poor sites contain subsets of species-rich ones. Here, we examine nested patterns across the macroinvertebrate assemblages of 91 ponds in Doñana National Park, Spain, and explore temporal variation of nestedness and species richness in 19 temporary ponds over 2 years with differing rainfall. Macroinvertebrate assemblages were significantly nested; both pond spatial arrangement and environmental variation being important in driving nested patterns. Despite the nested structure observed, a number of taxa and ponds deviate from this pattern (termed idiosyncratic), by occurring more frequently than expected in species-poor sites, or having assemblages dominated by species largely absent from species-rich sites. Aquatic adults of winged insects, capable of dispersal, were more highly nested than non-dispersing taxa and life-history stages. Idiosyncratic taxa were found in ponds spanning a wide range of hydroperiods, although nestedness was higher in more permanent waterbodies. Monthly sampling demonstrated a gradual increase of species richness and nestedness from pond filling to April–May, when the most temporary ponds started to dry. Although the degree of nestedness of individual pond assemblages varied from month to month, the overall degree of nestedness in the two study years was practically identical despite marked differences in hydroperiod. Our results suggest that differential colonization and environmental variation are key processes driving the nested structure of Doñana ponds, that macroinvertebrate assemblages change in a predictable manner each year in response to cycles of pond wetting and drying, and that connectivity and environmental variability maintain biodiversity in pond networks. 相似文献
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Copepods and branchiopods of temporary ponds in the Doñana Natural Area (SW Spain): a four-decade record (1964–2007) 总被引:1,自引:1,他引:0
The Doñana Natural Area includes a large array of wetlands with the highest degree of environmental protection in Spain, and so it has long attracted many studies. We present a cumulative list of zooplankton taxa (Copepods and Branchiopods) based on a collection of 18 publications (1964–2007) and 4 unpublished studies. Seventy-eight taxa have been recorded in a set of 55 ponds, and 72 taxa at 38 sites spread over the Doñana marshland. In total, 96 taxa have been recorded, including 50% of all branchiopod species reported for the whole Iberian Peninsula. Taxa composition was significantly segregated between ponds and marshland during floods (ANOSIM test, R = 0.929, P < 0.01), but this segregation disappeared at a larger spatio-temporal scale when a non-metric MDS ordination produced a gradient from ponds to marshland (ANOSIM test, R = 0.272, P < 0.01). The lack of segregation between ponds and marshland sites, and among ponds with different hydroperiods, was not due to a large number of cosmopolitan species, but to a random distribution of a large number of low-occurrence species (67% of total taxa occurred with a frequency <15%). Long-hydroperiod ponds occupy a key position among the Doñana wetlands in terms of biodiversity as these ponds accumulated a high crustacean richness over time. They also supported a significantly higher cumulative number of cladoceran and harpacticoid taxa, while short-hydroperiod ponds accumulated the lowest number of diaptomid taxa. Our data indicate the need for recording biodiversity in the long term as richness on a short-temporal scale is not a good indicator of the number of crustacean species that would be encountered with a longer sampling period in Mediterranean temporary wetlands. 相似文献
18.
Rodrigo Mendonça de Lucena Sávia Gavazza Lourdinha Florencio Mario T. Kato Marcos Antonio de MoraisJr 《World journal of microbiology & biotechnology》2011,27(12):2893-2902
The microorganisms diversity in a full-scale UASB reactor treating domestic sewage was studied by molecular techniques, with
the objective of identifying the population differences associated with the specific methanogenic activity (SMA) of the sludges.
Samples were collected at levels A (0.8 m; bottom), B (1.3 m), C (1.8 m), D (2.3 m) and E (2.8 m). Actinobacteria was dominant at the three lower points and should have been primarily responsible for the degradation of organic matter.
DNA sequences belonging to Methanomicrobiales order of Archaea domain was detected in all five levels with the majority producing methane from hydrogen and carbon dioxide. Points A and
E showed similar bacteria variety. The SMA of point A was the highest (0.374 g COD-CH4/g SSV.d); however, the point E showed much lower value, probably due to the predominance of Proteobacteria phylum, including sulfate-reducing bacteria. In the overall, the results obtained can be considered important because data
from full-scale UASB reactors treating domestic sewage remain scarce. 相似文献
19.
Storgaard M Henriksen ST Zaragoza F Peschke B Tanner D 《Bioorganic & medicinal chemistry letters》2011,21(13):3918-3922
Herein is described the synthesis of a novel class of peptidyl FVIIa inhibitors having a C-terminal benzyl ketone group. This class is designed to be potentially suitable as stabilization agents of liquid formulations of rFVIIa, which is a serine protease used for the treatment of hemophilia A and B inhibitor patients. A library of compounds was synthesized with different tripeptide sequences, N-terminals and d-amino acids in the P3 position. Cbz-d-Phe-Phe-Arg-bk (33) was found to be the best candidate with a potency of Ki = 8 μM and no substantial inhibition of related blood coagulation factors (thrombin and FXa). Computational studies revealed that 33 has a very stable binding conformation due to intramolecular hydrogen bonds, which cannot be formed with l-Phe in the P3 position. Nonpolar amino acids were found to be superior, probably due to a minimization of the cost of desolvation upon binding to FVIIa. 相似文献
20.
Carla V. Galmozzi Lorena Saelices Francisco J. Florencio M. Isabel Muro-Pastor 《Journal of bacteriology》2010,192(18):4701-4711